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Biochem. J. 80, 254. , Stallberg-Stenhagen, S. and Stenhagen, E. (1959). Ada Chem. Scand. 13, 822. Asselineau, J. and Lederer, E. (1955). "Ciba Found. Symp. Exp. Tuberc. , Boston, p. 14. Asselineau, J. and Lederer, E. (1960). In "Lipid Metabolism" (K. ). John Wiley and Sons, New York, p. 337. Astbury, W. , Beighton, E. and Weibull, C. (1955). Symp. Soc. Exptl Biol. 9, 282. , Buchanan, J. , Martin, R. , and Rajbhandary, U. L. (1962). Biochem. J. 85, 49. Ball, D. H. and Adams, G. A. (1959). Can. J.

There has always been an active interest in the chemistry of the tubercle bacillus with efforts to relate the lipids of this organism to the pathogenesis of tuberculosis and the acid-fast staining properties of this organism (Asselineau and Lederer, 1955). A considerable body of information has resulted concerning the chemistry of Mycobacterium species, but the original problems remain unsolved. The earliest fruitful chemical studies were by Anderson and Chargaff (1929), when they isolated tuberculostearic acid from the tubercle bacillus (Fig.

With the aid of kinetic studies, he determined t h a t Myrothecium cellulase (ß- 1,4-glucan 4-glucanohydrolase) attacked the substrate at random points along the cellulose chain to produce both cellobiose and glucose as the products. Several pieces of evidence indicated t h a t the observed enzymic activity was due to the action of a single enzyme. On the other hand, Cellvibrio gilvus, an organism isolated from cow manure, produces a cellulase with a mode of action analogous to j8amylase. Storvick et dl.

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