By K. Eric Wommack, Kurt E. Williamson, Rebekah R. Helton, Shellie R. Bench (auth.), Martha R.J. Clokie, Andrew M. Kropinski (eds.)
Ranging from the evolution of pathogenicity to oceanic carbon biking, the numerous and sundry roles that bacteriophages play in microbial ecology and evolution have encouraged elevated curiosity in the medical neighborhood. Bacteriophages: equipment and Protocols pulls jointly the gigantic physique of data and services from best foreign bacteriophage researchers to supply either classical and cutting-edge molecular recommendations. With its well-organized modular layout, Volume 1: Isolation, Characterization, and Interactions examines a mess of issues, together with the isolation of phages, morphological and molecular characterization, and interplay with micro organism. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters encompass short introductions to the topic, lists of the mandatory fabrics and reagents, effectively reproducible laboratory protocols, and a Notes part which info tips about troubleshooting and warding off identified pitfalls.
Thorough and state of the art, Bacteriophages: equipment and Protocols is a necessary reference for knowledgeable bacteriophage researchers in addition to an simply obtainable advent for beginners to the subject.
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Extra info for Bacteriophages: Methods and Protocols, Volume 1: Isolation, Characterization, and Interactions
6. C. N. E. F. Hatfull. (1999). Evolutionary relationships among diverse bacteriophages and prophages: all the world’s a phage. Proc. Natl. Acad. Sci. USA 96, 2192–2197. 7. Moreira, D. (2000). Multiple independent horizontal transfers of informational genes from bacteria to plasmids and phages: implications for the origin of bacterial replication machinery. Mol. Microbiol. 35: 1–5. 8. F. (2005). Bacteriophages and bacterial virulence, in Bacteriophages: Biology and Applications, (Kutter E. ), CRP Press, FL, pp.
A 1:10 dilution) and mix thoroughly by inversion (Notes 9 and 10). Fig. 1. Spot test on phage on a bacterial streak (left boxed area). A control, in which broth was spotted on the streak, is shown on the right. Photograph kindly provided by Erika J. Lingohr (PHAC-LFZ). Bacteriophage Enrichment from Water and Soil 19 3. Allow the sample to incubate at room temperature for at least 1 h to allow free phage to be suspended in the liquid component. Invert frequently during the incubation period to increase the disruption of particulate material and to distribute the phage throughout the solution.
Pour the water-saturated agar into the Buchner funnel. Turn on the vacuum pump. The water should be sucked from the agar. Maintain a vacuum of approximately 40–50 cm Hg. The agar should start to turn white in colour as the water is removed. Keep the pump on until there is no longer a steady drip of water from the Buchner funnel (Note 5). Use a spatula to loosen the agar from the edges of Buchner funnel and tip the agar back into the 5 l beaker. Add 5 l of water and repeat the washing procedure (steps 3–9) until the water being removed runs clear.