By David G. Lambert

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Morgan, 1998 78. Antibacterial Peptide Protocols, edited by William M. Shafer, 1997 77. Protein Synthesis: Methods and Protocols, edited by Robin Martin, 1998 76. Glycoanalysis Protocols (2nd. ), edited by Elizabeth F. Hounsell, 1998 75. Basic Cell Culture Protocols (2nd. ), edited by Jeffrey W. Pollard and John M. Walker, 1997 74. Ribozyme Protocols, edited by Philip C. Turner, 1997 73. Neuropeptide Protocols, edited by G. Brent Irvine and Carvell H. Williams, 1997 72. Neurotransmitter Methods, edited by Richard C.

Thus, Ca2+ can be monitored simultaneously with other physiologically important ions such as Na+ or H+ (1719). Moreover, Ca2+ can be monitored using indicators in separate domains as with simultaneous measurements of intracellular and extracellular Ca2+ (20). Page 6 Table 1 Single Excitation Wavelength IndicatorsAbsorbanceEmissionIndicatorSourceaUV/VKdAM loadingCa2++Ca2+Ca2++Ca2+CommentsQuin2MP/TLUV60b(115)d352332492498High intracellular bufferingMethoxyquin2MFMPUV65b352332492498 MethoxyquinMF19 for nuclear magnetic resonance Oregon Green 488 BAPTA-1 MPV170b494494523523Designed for argon-ion lasersCalcium OrangeäMPV185b(380)e549549575576See ref.

25]. Printed in the United States of America. 10 9 8 7 6 5 4 3 2 1 Library of Congress Cataloging in Publication Data Main entry under title: Methods in molecular biologyä.  Lambert p.  114) Includes index.  Lambert, David G.  Series. 516dc21 98-44482 CIP Page v PREFACE The regulation of intracellular Ca2+ is a common theme presented in many papers over the last 20 or so years, and the description of the Ca2+-sensitive indicator dye, fura 2, in 1985 resulted in a massive increase in these types of studies.

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