By Frank H. Stephenson
Vital for all Biotechnology scholars and technicians. It walks readers during the minefield of laboratory calculations, assisting to avoid the kinds of blunders which may suggest the lack of weeks of labor. Key Features:* issues variety from easy medical notations to complicated topics like nucleic acid chemistry and recombinant DNA know-how* each one bankruptcy contains a short clarification of the idea that and covers worthy definitions, concept and motive for every kind of calculation* contemporary functions of the approaches and computations in scientific, educational, commercial and uncomplicated examine laboratories are mentioned during the textNew to this Edition:* up to date and elevated insurance of genuine time PCR and the math used to degree gene expression* extra pattern difficulties in each bankruptcy for readers to perform innovations
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Additional info for Calculations for Molecular Biology and Biotechnology, Second Edition: A Guide to Mathematics in the Laboratory 2e
Multiply both sides of the equation by 100. Simplify. Subtract 50x from both sides of the equation (Addition Property of Equality). Divide both sides of the equation by 1950. Convert mL to L and round off to one significant figure to the right of the decimal point. 5385 mL. 5 mL, the concentration of that component would change by the addition of the SDS. 2%, its concentration would be altered 24 CHAPTER 2 Solutions, mixtures, and media by the addition of more liquid. 003 g of NaCl. 023 1023 molecules.
14. 14 parts. 0. n Chapter summary Concentration is defined as an amount of some substance per a set volume: concentration amount volume Dilutions can be calculated using the C1V1 C2V2 equation or by the dimensional analysis method. The molecular weight of a compound is calculated as the sum of the atomic weights of its component molecules. A 1 molar (1 M) solution of a chemical contains the molecular weight of the substance dissolved in a total volume of 1 L. 015 mL water mL water from hydrated compound where V is the final volume in liters, M is the desired molarity (in moles/ liter), and ‘molecules of water’ is the number of water molecules attached to the compound.
3 Preparing percent solutions Many reagents are prepared as a percent of solute (such as salt, cesium chloride, or sodium hydroxide) dissolved in solution. ’ 12%, therefore, means 12 per 100, or 12 out of every 100. 12). Depending on the solute’s initial physical state, its concentration can be expressed as a weight per volume percent (% w/v) or a volume per volume percent (% v/v). A percentage in weight per volume refers to the weight of solute (in grams) in a total of 100 mL of solution. A percentage in volume per volume refers to the amount of liquid solute (in mL) in a final volume of 100 mL of solution.