By Mitchelson, Cheng
An excellent panel of hands-on specialists and builders of CE apparatus describe in step by step style their most sensible state-of-the-art tools for the detection and research of DNA mutations and transformations, starting from specified DNA loci to complete genomes of organisms. this primary quantity of the set, advent to the Capillary Electrophoresis of Nucleic Acids, covers the sensible and theoretical issues in the back of using capillary electrophoresis for the research of small oligonucleotides and converted nucleotides. in addition to targeted directions making sure prepared reproducibility, those protocols provide time-tested suggestion on instrumentation, sign detection, the capillary atmosphere, and the combination of mass spectrometry with CE. numerous chapters are dedicated to the research of small healing oligonucleotides, nucleosides, and ribonucleotides by way of CE. The spouse quantity, useful purposes of Capillary Electrophoresis, addresses options for high-throughput research of DNA fragments utilizing SNP detection, mutation detection, DNA sequencing equipment, and DNA-ligand interactions. accomplished and up to date, the paired volumes of Capillary Electrophoresis of Nucleic Acids provide an authoritative advisor with quick access to speedy, flexible, trustworthy, and robust applied sciences for all these simple and scientific investigators studying DNA edition this present day.
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Extra info for Capillary Electrophoresis of Nucleic Acids Volume 1 Introduction to the Capillary Electrophoresis (Methods in Molecular Biology Vol 162)
49–56. 42. , and Yeung, E. S. (1999) Automated and integrated system for highthroughput DNA genotyping directly from blood. Anal. Chem. 71, 1138–1145. 43. Mansfield, E. , Wilson, R. , and Fortina, P. (2001) Analysis of short tandem repeat markers by capillary array electrophoresis, in Capillary Electrophoresis of Nucleic Acids, Vol. 2 (Mitchelson, K. R. ), Humana Press, Totowa, NJ, pp. 151–161. 44. Mansfield, E. , Robertson, J. , Isenberg, A. , Frazier, R. , Harris, D. , Barker, D. , Gill, P. , and McCord, B.
1. Enzyme Mismatch Cleavage Enzyme mismatch cleavage (EMC) creates a DNA fragment length polymorphism by using bacteriophage T4 endonuclease VII (57) or Cleavase (58) to cleave heteroduplex DNAs at single-nucleotide mismatches and small heteroduplex loops. The majority of the possible mismatch combinations can be rapidly identified by cleavage 12 Mitchelson scanning of 1-kbp fragments of DNA. Preliminary evidence indicates that crude PCR products can be analyzed directly by EMC. Cleavase nuclease (58) and CE analysis of the DNA cleavage pattern have been used to detect mutations in the human genes, and may prove to be a useful system for automated, large-scale genetic screening.
1996) Design and optimization of a capillary electrophoretic mobility shift assay involving trp repressorDNA complexes. J. Chromatogr. B 683, 77–84. 103. , Gabrielsen, O. , and Myrset, A. H. (1997) Capillary electrophoretic mobility shift assay (CEMSA) a protein-DNA complex. J. Capillary Electrophor. 4, 225–231. 104. Foulds, G. J. and Etzkorn, F. A. (2001) Protein-DNA binding affinities by capillary electrophoresis, in Capillary Electrophoresis of Nucleic Acids, Vol. 2 (Mitchelson, K. R. ), Humana Press, Totowa, NJ, pp.