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Extra resources for Case Files: Biochemistry (Lange Case Files), 2nd edition
E. It is integrated into the host cell genome. 2] CASE FILES: BIOCHEMISTRY If a double-stranded DNA molecule undergoes two rounds of replication in an in vitro system that contains all of the necessary enzymes and nucleoside triphosphates that have been labeled with 32P, which of the following best describes the distribution of radioactivity in the four resulting DNA molecules? A. Exactly one of the molecules contains no radioactivity. B. Exactly one of the molecules contains radioactivity in only one strand.
This study reveals a new concept about nucleoside analogs, that is, mutagenic ribonucleosides may represent an important class of anti-RNA virus agents. RNA viruses like HIV and the influenza virus use a naturally high mutation rate of their RNA polymerases with low fidelity for replication to avoid and escape most treatments and vaccines. A RNA virus tends to mistakenly insert ribavirin into newly synthesized copies of their RNA genome. But ribavirin adds so many extra mutations to the virus during the replication of its genetic materials, it induces a flood of mutations and thus pushes the virus into a kind of genetic meltdown.
It uses this as a primer that is elongated by the polymerase to synthesize the viral (+)-mRNA using the (−)-viral RNA genome as the template. 2] C. After two rounds of replication using 32P-labeled nucleoside triphosphates (NTPs), all four DNA molecules will be radioactive; two will be radioactive in both strands, two will be radioactive in only one strand. 3] D. Xeroderma pigmentosum is a genetic disease in which the ability to remove pyrimidine dimers caused by exposure to UV light is impaired.